ABSTRACT
Novel mRNA vaccines have resulted in a reduced number of SARS-CoV-2 infections and hospitalizations. Yet, there is a paucity of studies regarding their effectiveness on immunocompromised autoimmune subjects. In this study, we enrolled subjects naive to SARS- CoV-2 infections from two cohorts of healthy donors (HD, n=56) and systemic lupus erythematosus (SLE, n=69). Serological assessments of their circulating antibodies revealed a significant reduction of potency and breadth of neutralization in the SLE group, only partially rescued by a 3rd booster dose. Immunological memory responses in the SLE cohort were characterized by a reduced magnitude of spike-reactive B and T cell responses that were strongly associated with poor seroconversion. Vaccinated SLE subjects were defined by a distinct expansion and persistence of a DN2 spike-reactive memory B cell pool and a contraction of spike-specific memory cTfh cells, contrasting with the sustained germinal center (GC)-driven activity mediated by mRNA vaccination in the healthy population. Among the SLE-associated factors that dampened the vaccine responses, treatment with the monoclonal antibody anti-BAFF/Belimumab (a lupus FDA- approved B cell targeting agent) profoundly affected the vaccine responsiveness by restricting the de novo B cell responses and promoting stronger extra-follicular (EF)-mediated responses that were associated with poor immunogenicity and impaired immunological memory. In summary, this study interrogates antigen-specific responses and characterized the immune cell landscape associated with mRNA vaccination in SLE. The identification of factors associated with reduced vaccine efficacy illustrates the impact of SLE B cell biology on mRNA vaccine responses and provides guidance for the management of boosters and recall vaccinations in SLE patients according to their disease endotype and modality of treatment.
Subject(s)
Memory Disorders , Severe Acute Respiratory Syndrome , Lupus Erythematosus, SystemicABSTRACT
The Vietnamese government has issued several directives of lockdown in Hanoi, the capital city, for a month (1–30 April 2020) to prevent the human-to-human transmission of COVID-19. This action has affected air pollution due to a decline in transportation. Therefore, this study investigates spatial and temporal change in the PM2.5 concentrations during the first 4 months of 2020 in Hanoi metropolitan area. Spatial distribution maps of the PM2.5 concentration in Hanoi were provided for the first time. The average PM2.5 concentrations at the 22 air monitoring stations were strongly correlated with the population. April had a significantly lower level of PM2.5 than the other 3 months. In particular, the concentrations of PM2.5 and NO2 decreased by 12 and 54%, respectively, between March and April, especially at areas for commercial activities. In April, a higher level of PM2.5 was recorded between Tuesday–Thursday, which is a reverse trend with that in March. Furthermore, Monday and Friday did not show rush hour peaks for the PM2.5 levels in April. A decrease in the PM2.5 concentrations was partly influenced by the long-range transport from the outside to Hanoi. This study implies that a reduction in traffic volumes and public activities may possibly improve the air quality. [ABSTRACT FROM AUTHOR] Copyright of International Journal of Environmental Analytical Chemistry is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)